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STEMCELL Technologies Inc easy-sep rosette human cd4 + t-cell enrichment kit
Easy Sep Rosette Human Cd4 + T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easy+sep+human+cd4++t+cell+enrichment+kit/cd4++rosette+kit/pmc07927817-336-15-23
Average 90 stars, based on 1 article reviews
easy-sep rosette human cd4 + t-cell enrichment kit - by Bioz Stars, 2026-09
90/100 stars

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Isolation:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Flow Cytometry:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Staining:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Activation Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Negative Control:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Expressing:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Control:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Derivative Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Selection:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Fluorescence:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Cell Isolation:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Marker:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Viability Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Concentration Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Standard Deviation:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Infection:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Whisker Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Cell Cycle Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Software:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

RNA Sequencing Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Transformation Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Single Vesicle Fusion Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

RT Activity Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Activity Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Cell Culture:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Sample Prep:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Purification:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Labeling:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Liquid Chromatography with Mass Spectroscopy:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Functional Assay:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Western Blot:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Immunoprecipitation:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Comparison:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Plasmid Preparation:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Virus:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Inhibition:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Gene Expression:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

SDS Page:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).

Membrane:

Article Title: Epigenetic Compound Screening Uncovers Small Molecules for Reactivation of Latent HIV-1
Article Snippet: Content was diluted with PBS (Gibco) and CD4 + T cells were isolated via the Easy-Sep Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to the manufacturer’s instructions and purity of isolated CD4 + T cells was controlled on a FACSverse flow cytometer (BD Biosciences) using BD Tritest CD4/CD8/CD3 antibody stain (BD Biosciences).

Article Title: Expression of soluble programmed death-1 protein in peripheral blood regulatory T cells and its effects on rheumatoid arthritis progression.
Article Snippet: PBMCs from the study subjects, obtained by Ficoll density gradient separation, were used for the positive selection of human CD4+CD25- Tregs with the Easy Sep Human CD4+ T cell Enrichment kit (Stemcell Technologies, Inc., Vancouver, BC, Canada).

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells
Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-SepTM Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Article Title: An Integrated Genomic and Transcriptomic Analysis Reveals Candidates of Susceptibility Genes for Crohn’s Disease in Japanese Populations
Article Snippet: CD4+ T cells were isolated by negative selection from isolated LPMCs using an Easy Sep Magnet (STEMCELL Technology, Vancouver, Canada) and an Easy Sep Human CD4+ T cell Enrichment kit (STEMCELL Technology).



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STEMCELL Technologies Inc easy-sep tm rosette human cd4+ t cell enrichment kit
Easy Sep Tm Rosette Human Cd4+ T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
easy-sep tm rosette human cd4+ t cell enrichment kit - by Bioz Stars, 2026-09
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STEMCELL Technologies Inc easy sep human cd4+ t cell enrichment kit
Easy Sep Human Cd4+ T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easy+sep+human+cd4++t+cell+enrichment+kit/cd4+++t+cell+isolation+kit/pmc07314801-202-22-30
Average 90 stars, based on 1 article reviews
easy sep human cd4+ t cell enrichment kit - by Bioz Stars, 2026-09
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STEMCELL Technologies Inc easy-sep™ rosette human cd4 + t-cell enrichment kit
A) <t>CD4</t> + T-cells were isolated from blood of healthy donors using RosetteSep™ technology. Subsequently, purity of isolated cells was analyzed by fluorescence-based flow cytometry using anti-CD3 / -CD4 staining. FACS plots display the gating strategy for living cells (lymphocytes; left), singlets (middle), and enriched CD4 + T-cells (right). Numbers display the percentage of cells within the respective gate. B) Representative FACS plots of CD4 + T-cell isolation based on the gating strategy in A from four different donors, which were used later in four-donor-pools. C) Schematic illustration of T-cell activation. Isolated CD4 + T-cells (resting) were cultivated in the presence of PHA and IL-2 for 5-8 days to gain activated CD4 + T-cells. D) Representative FACS plots of the isolated cells from B after activation. CD69 was used as activation marker.
Easy Sep™ Rosette Human Cd4 + T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easy+sep+human+cd4++t+cell+enrichment+kit/cd4++rosette+kit/bio_rxiv__2020__04__29__067884-146-19-27
Average 90 stars, based on 1 article reviews
easy-sep™ rosette human cd4 + t-cell enrichment kit - by Bioz Stars, 2026-09
90/100 stars
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90
STEMCELL Technologies Inc easy sep cd4 + human t cell enrichment kit
Effects of Th17-polarizing cytokines on the percentage of HIV-infected, <t>CD4</t> T cells. (A) Human CD4+ T cells were isolated from peripheral blood by negative selection and then mitogenically activated with phytohemagglutinin (PHA; 5 μg/ml) and interleukin-2 (IL-2; 10 ng/ml) for 3 days prior to infection. Cells were then washed, infected with the indicated isolates of HIV for 2.5 h, washed again, and then resuspended in medium containing IL-2 or the Th17-polarizing cytokines IL-1β, IL-6, and IL-23 (10 ng/ml) and TGF-β (2 ng/ml). (B and C) Time course (B) and day 5 comparison (C) of the total percentages of p24+ cells from IL-2 and from Th17-polarizing conditions. D0, D3, D5, and D7, days 0, 3, 5, and 7, respectively. (D) Representative plots of cells infected for 5 days with CCR5-tropic HIVBaL, CXCR4-tropic HIVNL4-3, or CCR5-tropic, transmitted-founder isolate HIVAD17. (E) The indicated cytokines were added after infection with HIVBaL. The IL-17 expression shown was in uninfected samples. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001.
Easy Sep Cd4 + Human T Cell Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easy+sep+human+cd4++t+cell+enrichment+kit/cd4+++t+cell+isolation+kit/pmc04988157-59-17-31
Average 90 stars, based on 1 article reviews
easy sep cd4 + human t cell enrichment kit - by Bioz Stars, 2026-09
90/100 stars
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A) CD4 + T-cells were isolated from blood of healthy donors using RosetteSep™ technology. Subsequently, purity of isolated cells was analyzed by fluorescence-based flow cytometry using anti-CD3 / -CD4 staining. FACS plots display the gating strategy for living cells (lymphocytes; left), singlets (middle), and enriched CD4 + T-cells (right). Numbers display the percentage of cells within the respective gate. B) Representative FACS plots of CD4 + T-cell isolation based on the gating strategy in A from four different donors, which were used later in four-donor-pools. C) Schematic illustration of T-cell activation. Isolated CD4 + T-cells (resting) were cultivated in the presence of PHA and IL-2 for 5-8 days to gain activated CD4 + T-cells. D) Representative FACS plots of the isolated cells from B after activation. CD69 was used as activation marker.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: A) CD4 + T-cells were isolated from blood of healthy donors using RosetteSep™ technology. Subsequently, purity of isolated cells was analyzed by fluorescence-based flow cytometry using anti-CD3 / -CD4 staining. FACS plots display the gating strategy for living cells (lymphocytes; left), singlets (middle), and enriched CD4 + T-cells (right). Numbers display the percentage of cells within the respective gate. B) Representative FACS plots of CD4 + T-cell isolation based on the gating strategy in A from four different donors, which were used later in four-donor-pools. C) Schematic illustration of T-cell activation. Isolated CD4 + T-cells (resting) were cultivated in the presence of PHA and IL-2 for 5-8 days to gain activated CD4 + T-cells. D) Representative FACS plots of the isolated cells from B after activation. CD69 was used as activation marker.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Isolation, Fluorescence, Flow Cytometry, Staining, Cell Isolation, Activation Assay, Marker

Activated CD4 + T-cells were cultivated in the presence of different concentrations of the respective HDACIs for 72h. Afterwards, viability of cells was determined using a luminescence-based viability assay. Besides individual data points the fitting curve of all measurements, R 2 value, Hill slope (Hs), and lethal concentration of 20% (LC 20 ) is displayed. Data are derived from three independent experiments each with 2 independent four-donor pools. Error bars show standard deviation (SD). For Romidepsin the mean of three independent experiments as well as concentration-dependent viability curves of 4 individual donors are shown (upper left graph).

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: Activated CD4 + T-cells were cultivated in the presence of different concentrations of the respective HDACIs for 72h. Afterwards, viability of cells was determined using a luminescence-based viability assay. Besides individual data points the fitting curve of all measurements, R 2 value, Hill slope (Hs), and lethal concentration of 20% (LC 20 ) is displayed. Data are derived from three independent experiments each with 2 independent four-donor pools. Error bars show standard deviation (SD). For Romidepsin the mean of three independent experiments as well as concentration-dependent viability curves of 4 individual donors are shown (upper left graph).

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Viability Assay, Concentration Assay, Derivative Assay, Standard Deviation

A) Experimental design. Isolated, primary CD4 + T-cells were activated by PHA and IL-2 treatment for 5-8 days. Derived activated T-cells were treated with HDACIs for 24 h and subsequently infected with HIV-1 NL4-3 . 48 h post infection, cells were stained for the viral p24 capsid protein and analyzed by flow cytometry. B) Representative FACS histogram plot showing the increase of p24 capsid protein in infected cells (green). Uninfected cells were used as control (grey) C) Box-Whisker plots representing the HIV-1 NL4-3 infection levels in CD4 + T-cells in dependency of the respective HDACI treatment. All data were normalized to the median of vehicle control samples. Boxes represent the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Individual data points are presented as red dots. Sample size: n ≥ 10. Given p-values show statistically difference (ANOVA-based Dunnetts’ multiple comparison test) between vehicle control and sodium butyrate and bufexamac treated cells. D) Graph similar to C except infection with R5-tropic HIV-1 T/F CH058 virus. Sample size: n = 9.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: A) Experimental design. Isolated, primary CD4 + T-cells were activated by PHA and IL-2 treatment for 5-8 days. Derived activated T-cells were treated with HDACIs for 24 h and subsequently infected with HIV-1 NL4-3 . 48 h post infection, cells were stained for the viral p24 capsid protein and analyzed by flow cytometry. B) Representative FACS histogram plot showing the increase of p24 capsid protein in infected cells (green). Uninfected cells were used as control (grey) C) Box-Whisker plots representing the HIV-1 NL4-3 infection levels in CD4 + T-cells in dependency of the respective HDACI treatment. All data were normalized to the median of vehicle control samples. Boxes represent the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Individual data points are presented as red dots. Sample size: n ≥ 10. Given p-values show statistically difference (ANOVA-based Dunnetts’ multiple comparison test) between vehicle control and sodium butyrate and bufexamac treated cells. D) Graph similar to C except infection with R5-tropic HIV-1 T/F CH058 virus. Sample size: n = 9.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Isolation, Derivative Assay, Infection, Staining, Flow Cytometry, Whisker Assay

A and B) Volcano plots of identified transcripts showing the log 2 fold change of abundance between inhibitor (A: Sb, B: Bu) and vehicle control and the associated q-value for each transcript. Colour code: Red indicates significantly upregulated transcripts (FDR ≤ 001 and log 2 fold change ≥ 1), Blue indicates significantly downregulated transcripts (FDR ≤ 001 and log 2 fold change ≤ −1), and Grey indicates significantly differential expressed genes, which are not up- or down-regulated (FDR ≤ 001). C). GO-term and pathway enrichment analysis of significantly down-regulated genes in Sb- (left) and Bu-treated cells (right) based on the g:profiler analysis tool. Representative terms are shown. The graphs display q-values and amount of regulated genes for each indicated GO-term and KEGG pathway. See also Figure S6C. D) Cell cycle analysis. Jurkat-E6 cells were treated with Sb or Bu for 48 h. Afterwards, cells were fixed, stained with propidium iodide (PI) and analyzed by flow cytometry. Cell cycle phases were determined using FlowJo software. Histogramms on the left show representative measurements. Donut charts on the right display the amount of cells in the different cell cycle phases as derived from 3 independent experiments. E) Determination of 2-LTR circles. Graph displays the percentage of HIV-1-based 2-LTR circles measured in compound-treated CD4 + T-cells. Romidepsin (Ro) was used as internal control. Data are derived from 3 independent experiments and are normalized to vehicle-treated control cells. Error bars display SD. Statistics was performed using the ANOVA-based Tukeys’ multiple comparison test.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: A and B) Volcano plots of identified transcripts showing the log 2 fold change of abundance between inhibitor (A: Sb, B: Bu) and vehicle control and the associated q-value for each transcript. Colour code: Red indicates significantly upregulated transcripts (FDR ≤ 001 and log 2 fold change ≥ 1), Blue indicates significantly downregulated transcripts (FDR ≤ 001 and log 2 fold change ≤ −1), and Grey indicates significantly differential expressed genes, which are not up- or down-regulated (FDR ≤ 001). C). GO-term and pathway enrichment analysis of significantly down-regulated genes in Sb- (left) and Bu-treated cells (right) based on the g:profiler analysis tool. Representative terms are shown. The graphs display q-values and amount of regulated genes for each indicated GO-term and KEGG pathway. See also Figure S6C. D) Cell cycle analysis. Jurkat-E6 cells were treated with Sb or Bu for 48 h. Afterwards, cells were fixed, stained with propidium iodide (PI) and analyzed by flow cytometry. Cell cycle phases were determined using FlowJo software. Histogramms on the left show representative measurements. Donut charts on the right display the amount of cells in the different cell cycle phases as derived from 3 independent experiments. E) Determination of 2-LTR circles. Graph displays the percentage of HIV-1-based 2-LTR circles measured in compound-treated CD4 + T-cells. Romidepsin (Ro) was used as internal control. Data are derived from 3 independent experiments and are normalized to vehicle-treated control cells. Error bars display SD. Statistics was performed using the ANOVA-based Tukeys’ multiple comparison test.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Cell Cycle Assay, Staining, Flow Cytometry, Software, Derivative Assay

A) Correlation plot of RNA-Seq data from Sb- and Bu-treated cells. Only transcripts are shown, which could be assigned to an ENSEMBL ID. DEGs with log 2 fold change > −1 and < 1 are depicted in grey. Regulated DEGs (log 2 fold change ≥ 1 or ≤ −1) are indicated as follows: upregulated in only one condition (orange), upregulated in both conditions (red), downregulated in only one condition (light blue), downregulated in both conditions (dark blue), and contrary regulated (magenta). n = number of DEGs belonging to the respective group. B) Heatmaps showing the 20 most up- or down-regulated genes in Sb-treated (left) and Bu-treated (right) cells. Colour intensity shows row z-score of variance stabilized transformation (VST) values. Top 20 genes that have been identified in both treatment conditions are indicated with red letters. C) GO-term-analysis of up-regulated genes. Left: Sb-treatment, Right: Bu-treatment. Representative terms are shown. The graphs display q-value and amount of regulated genes for each indicated GO-term. See also . D) Viral particle fusion assay. Box-Whisker plot displays the percentage of fusion of viral particles with the target cell. Datasets were normalized to untreated control cells and were derived from three independent experiments. Boxes show the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Statistics was performed using one-way ANOVA-based Dunnetts’ multiple comparison test. Sb: Sodium Butyrate, Bu: Bufexamac, Ro: Romidepsin. E) RT activity assay. Graph displays RT activity in dependency of HDACI treatment as analyzed in HIV-1 infected CD4 + T-cells. Data were derived from four independent experiments; error bars show SD.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: A) Correlation plot of RNA-Seq data from Sb- and Bu-treated cells. Only transcripts are shown, which could be assigned to an ENSEMBL ID. DEGs with log 2 fold change > −1 and < 1 are depicted in grey. Regulated DEGs (log 2 fold change ≥ 1 or ≤ −1) are indicated as follows: upregulated in only one condition (orange), upregulated in both conditions (red), downregulated in only one condition (light blue), downregulated in both conditions (dark blue), and contrary regulated (magenta). n = number of DEGs belonging to the respective group. B) Heatmaps showing the 20 most up- or down-regulated genes in Sb-treated (left) and Bu-treated (right) cells. Colour intensity shows row z-score of variance stabilized transformation (VST) values. Top 20 genes that have been identified in both treatment conditions are indicated with red letters. C) GO-term-analysis of up-regulated genes. Left: Sb-treatment, Right: Bu-treatment. Representative terms are shown. The graphs display q-value and amount of regulated genes for each indicated GO-term. See also . D) Viral particle fusion assay. Box-Whisker plot displays the percentage of fusion of viral particles with the target cell. Datasets were normalized to untreated control cells and were derived from three independent experiments. Boxes show the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Statistics was performed using one-way ANOVA-based Dunnetts’ multiple comparison test. Sb: Sodium Butyrate, Bu: Bufexamac, Ro: Romidepsin. E) RT activity assay. Graph displays RT activity in dependency of HDACI treatment as analyzed in HIV-1 infected CD4 + T-cells. Data were derived from four independent experiments; error bars show SD.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: RNA Sequencing Assay, Transformation Assay, Single Vesicle Fusion Assay, Whisker Assay, Derivative Assay, RT Activity Assay, Activity Assay, Infection

CD4 + T-cells were treated with bufexamac in presence of increasing concentrations of iron (FeCl 3 ). 24 h post treatment, cells were infected with HIV-1 NL4-3 and further cultured for another 48 h. Subsequently, cells were analyzed for p24 via flow cytometry. Untreated cells were used for control.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: CD4 + T-cells were treated with bufexamac in presence of increasing concentrations of iron (FeCl 3 ). 24 h post treatment, cells were infected with HIV-1 NL4-3 and further cultured for another 48 h. Subsequently, cells were analyzed for p24 via flow cytometry. Untreated cells were used for control.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Infection, Cell Culture, Flow Cytometry

A) Schematic illustration of Sb-sample preparation. Cells were challenged for 48 h, lyzed, and subsequently proteins were digested by trypsin. Derived peptides were purified, labeled with isobaric TMT-tags, mixed and analyzed by LC-MS/MS. B) Dot plot displays all identified proteins and the associated log 2 fold change upon Sb-treament in comparison to control cells. Colored areas represent cut-offs for up-regulation (red; ≥ 2 x SD), down-regulation (blue; ≤ 2 x SD), and no regulation (≤ 2x SD ≥). Numbers (n) of regulated proteins are given on the upper (up-regulated) and lower (down-regulated) right side of the graph. Most changed proteins are indicated by protein name. C) Dot plot displays identified proteins in Bu-treated cells. Data were derived from and were reanalyzed according to similar thresholds as in B. D) Functional interaction network of regulated proteins in Sb-treated cells. Network analysis was performed using STRING database and networks were visualized via cytoscape. The color code displays the measured log 2 fold changes. Connected proteins within the network, which were down-regulated at protein but not at mRNA level are highlighted (bold black lines). E) Immunoblot of direct cell lysates from treated and HIV-1 infected CD4 + T-cells, respectively, as well as of co-immunoprecipitated samples, showing EP300 levels (left). Vinculin served as loading control. Graph on the right shows relative EP300 activity of enriched EP300 (IP samples). Boxes show the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Statistics was performed using the ANOVA-based Tukeys’ multiple comparison test. F) EP300-associated HIV-1 infection levels. Bars show HIV-1 NL4-3 infection levels of CD4 + T-cells treated either with sodium butyrate or the EP300 inhibitor anarcadic acid (10µM). Data are derived from four experiments and error bars show SD. G) Immunoblots of intracellular EP300 levels in control and Sb-treated cells in presence or absence of proteasome inhibitor MG-132.

Journal: bioRxiv

Article Title: Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cells

doi: 10.1101/2020.04.29.067884

Figure Lengend Snippet: A) Schematic illustration of Sb-sample preparation. Cells were challenged for 48 h, lyzed, and subsequently proteins were digested by trypsin. Derived peptides were purified, labeled with isobaric TMT-tags, mixed and analyzed by LC-MS/MS. B) Dot plot displays all identified proteins and the associated log 2 fold change upon Sb-treament in comparison to control cells. Colored areas represent cut-offs for up-regulation (red; ≥ 2 x SD), down-regulation (blue; ≤ 2 x SD), and no regulation (≤ 2x SD ≥). Numbers (n) of regulated proteins are given on the upper (up-regulated) and lower (down-regulated) right side of the graph. Most changed proteins are indicated by protein name. C) Dot plot displays identified proteins in Bu-treated cells. Data were derived from and were reanalyzed according to similar thresholds as in B. D) Functional interaction network of regulated proteins in Sb-treated cells. Network analysis was performed using STRING database and networks were visualized via cytoscape. The color code displays the measured log 2 fold changes. Connected proteins within the network, which were down-regulated at protein but not at mRNA level are highlighted (bold black lines). E) Immunoblot of direct cell lysates from treated and HIV-1 infected CD4 + T-cells, respectively, as well as of co-immunoprecipitated samples, showing EP300 levels (left). Vinculin served as loading control. Graph on the right shows relative EP300 activity of enriched EP300 (IP samples). Boxes show the lower and upper quartiles, whiskers show the minimum and maximal values, and the line inside the box indicates the median. Statistics was performed using the ANOVA-based Tukeys’ multiple comparison test. F) EP300-associated HIV-1 infection levels. Bars show HIV-1 NL4-3 infection levels of CD4 + T-cells treated either with sodium butyrate or the EP300 inhibitor anarcadic acid (10µM). Data are derived from four experiments and error bars show SD. G) Immunoblots of intracellular EP300 levels in control and Sb-treated cells in presence or absence of proteasome inhibitor MG-132.

Article Snippet: For isolation of CD4 + T-cells, blood cells were diluted with PBS (Gibco) and T-cells were isolated via the Easy-Sep™ Rosette Human CD4 + T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols.

Techniques: Sample Prep, Derivative Assay, Purification, Labeling, Liquid Chromatography with Mass Spectroscopy, Functional Assay, Western Blot, Infection, Immunoprecipitation, Activity Assay

Effects of Th17-polarizing cytokines on the percentage of HIV-infected, CD4 T cells. (A) Human CD4+ T cells were isolated from peripheral blood by negative selection and then mitogenically activated with phytohemagglutinin (PHA; 5 μg/ml) and interleukin-2 (IL-2; 10 ng/ml) for 3 days prior to infection. Cells were then washed, infected with the indicated isolates of HIV for 2.5 h, washed again, and then resuspended in medium containing IL-2 or the Th17-polarizing cytokines IL-1β, IL-6, and IL-23 (10 ng/ml) and TGF-β (2 ng/ml). (B and C) Time course (B) and day 5 comparison (C) of the total percentages of p24+ cells from IL-2 and from Th17-polarizing conditions. D0, D3, D5, and D7, days 0, 3, 5, and 7, respectively. (D) Representative plots of cells infected for 5 days with CCR5-tropic HIVBaL, CXCR4-tropic HIVNL4-3, or CCR5-tropic, transmitted-founder isolate HIVAD17. (E) The indicated cytokines were added after infection with HIVBaL. The IL-17 expression shown was in uninfected samples. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001.

Journal: Journal of Virology

Article Title: Human T h 17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection

doi: 10.1128/JVI.02869-15

Figure Lengend Snippet: Effects of Th17-polarizing cytokines on the percentage of HIV-infected, CD4 T cells. (A) Human CD4+ T cells were isolated from peripheral blood by negative selection and then mitogenically activated with phytohemagglutinin (PHA; 5 μg/ml) and interleukin-2 (IL-2; 10 ng/ml) for 3 days prior to infection. Cells were then washed, infected with the indicated isolates of HIV for 2.5 h, washed again, and then resuspended in medium containing IL-2 or the Th17-polarizing cytokines IL-1β, IL-6, and IL-23 (10 ng/ml) and TGF-β (2 ng/ml). (B and C) Time course (B) and day 5 comparison (C) of the total percentages of p24+ cells from IL-2 and from Th17-polarizing conditions. D0, D3, D5, and D7, days 0, 3, 5, and 7, respectively. (D) Representative plots of cells infected for 5 days with CCR5-tropic HIVBaL, CXCR4-tropic HIVNL4-3, or CCR5-tropic, transmitted-founder isolate HIVAD17. (E) The indicated cytokines were added after infection with HIVBaL. The IL-17 expression shown was in uninfected samples. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001.

Article Snippet: CD4 + T lymphocytes were isolated from human peripheral blood mononuclear cells by negative selection using the Easy Sep CD4 + human T cell enrichment kit, according to the manufacturer's protocol (Stem Cell Technologies Inc., Vancouver, BC, Canada).

Techniques: Infection, Isolation, Selection, Comparison, Expressing

Comparison of total and infected IL-17- or IFN-γ-producing cells. PHA-activated CD4+ T cells were infected with HIVBaL for 5 days in IL-2 or the Th17-polarizing cytokines IL-1β, IL-6, and IL-23 (10 ng/ml) and TGF-β (2 ng/ml). (A and B) Comparison of p24 expression among total, IL-17− IFN-γ−, IL-17+, and IL-17− IFN-γ+ T cells. (C and D) Comparison of IL-17 expression among total, p24−, or p24+ T cells. (E) Fold enrichment of p24+ cells among cells that either express or lack IFN-γ or IL-17. (F) Fold enrichment of IFN-γ- or IL-17-single-positive cells among p24+ cells, relative to p24− cells. (G) Percentages of IL-17- and IFN-γ-expressing cells were measured by flow cytometry. Blue depicts the proportion of IL-17− IFN-γ− cells, red shows IL-17+ cells, and yellow shows IFN-γ+ IL-17− cells. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001.

Journal: Journal of Virology

Article Title: Human T h 17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection

doi: 10.1128/JVI.02869-15

Figure Lengend Snippet: Comparison of total and infected IL-17- or IFN-γ-producing cells. PHA-activated CD4+ T cells were infected with HIVBaL for 5 days in IL-2 or the Th17-polarizing cytokines IL-1β, IL-6, and IL-23 (10 ng/ml) and TGF-β (2 ng/ml). (A and B) Comparison of p24 expression among total, IL-17− IFN-γ−, IL-17+, and IL-17− IFN-γ+ T cells. (C and D) Comparison of IL-17 expression among total, p24−, or p24+ T cells. (E) Fold enrichment of p24+ cells among cells that either express or lack IFN-γ or IL-17. (F) Fold enrichment of IFN-γ- or IL-17-single-positive cells among p24+ cells, relative to p24− cells. (G) Percentages of IL-17- and IFN-γ-expressing cells were measured by flow cytometry. Blue depicts the proportion of IL-17− IFN-γ− cells, red shows IL-17+ cells, and yellow shows IFN-γ+ IL-17− cells. *, 0.01 < P < 0.05; **, 0.001 < P < 0.01; ***, P < 0.001.

Article Snippet: CD4 + T lymphocytes were isolated from human peripheral blood mononuclear cells by negative selection using the Easy Sep CD4 + human T cell enrichment kit, according to the manufacturer's protocol (Stem Cell Technologies Inc., Vancouver, BC, Canada).

Techniques: Comparison, Infection, Expressing, Flow Cytometry

IL-17 and IFN-γ expression among CCR6-sorted, prepolarized cells infected with a pseudotype HIV vector, HIVAMLV. (A) Pie charts of average percentages of IL-17- and IFN-γ-expressing cells among CCR6-sorted cells that were Th0 or Th17 polarized and then infected with an Env-deficient HIV vector in an amphotropic murine leukemia virus (AMLV) envelope for 4 days. Total and p24+-gated cells from 5 donors are shown. (B) Flow cytometry plots from the donor whose cells became most Th17 polarized. (C) Representative plots of IL-17 and p24 expression in phytohemagglutinin (PHA)-activated CD4 T cells infected for 4 days with HIVAMLV in the presence of IL-2 or Th17-polarizing cytokines.

Journal: Journal of Virology

Article Title: Human T h 17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection

doi: 10.1128/JVI.02869-15

Figure Lengend Snippet: IL-17 and IFN-γ expression among CCR6-sorted, prepolarized cells infected with a pseudotype HIV vector, HIVAMLV. (A) Pie charts of average percentages of IL-17- and IFN-γ-expressing cells among CCR6-sorted cells that were Th0 or Th17 polarized and then infected with an Env-deficient HIV vector in an amphotropic murine leukemia virus (AMLV) envelope for 4 days. Total and p24+-gated cells from 5 donors are shown. (B) Flow cytometry plots from the donor whose cells became most Th17 polarized. (C) Representative plots of IL-17 and p24 expression in phytohemagglutinin (PHA)-activated CD4 T cells infected for 4 days with HIVAMLV in the presence of IL-2 or Th17-polarizing cytokines.

Article Snippet: CD4 + T lymphocytes were isolated from human peripheral blood mononuclear cells by negative selection using the Easy Sep CD4 + human T cell enrichment kit, according to the manufacturer's protocol (Stem Cell Technologies Inc., Vancouver, BC, Canada).

Techniques: Expressing, Infection, Plasmid Preparation, Virus, Flow Cytometry

Comparison of RNase expression and HIV inhibition in Th17-polarized cells with those in Th0-polarized cells. (A) Representative flow cytometry plots depicting IL-17 expression among Th0-polarized, CCR6− cells and Th17-polarized, CCR6+ cells. SSC, side scatter. (B) Box plot showing differential gene expression between Th0- and Th17-polarized cells. Total RNA from Th0- or Th17-polarized cells was analyzed by using microarrays. Relative levels of gene expression in Th17-polarized cells and Th0-polarized cells are shown. (C) Thirty micrograms of total protein from cell lysates of CCR6−, Th0-polarized and CCR6+, Th17-polarized CD4 T cells from three donors was denatured and separated according to size by SDS-PAGE, transferred to a PVDF membrane, and then probed for the indicated RNase expression with anti-RNase antibodies. The loading control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (D) Th0- and Th17-polarized CD4 T cells were infected with HIVIIIB, washed, and then resuspended in medium containing the indicated RNases. Percent inhibition was calculated from the percentage of p24+ cells, as measured by flow cytometry.

Journal: Journal of Virology

Article Title: Human T h 17 Cells Lack HIV-Inhibitory RNases and Are Highly Permissive to Productive HIV Infection

doi: 10.1128/JVI.02869-15

Figure Lengend Snippet: Comparison of RNase expression and HIV inhibition in Th17-polarized cells with those in Th0-polarized cells. (A) Representative flow cytometry plots depicting IL-17 expression among Th0-polarized, CCR6− cells and Th17-polarized, CCR6+ cells. SSC, side scatter. (B) Box plot showing differential gene expression between Th0- and Th17-polarized cells. Total RNA from Th0- or Th17-polarized cells was analyzed by using microarrays. Relative levels of gene expression in Th17-polarized cells and Th0-polarized cells are shown. (C) Thirty micrograms of total protein from cell lysates of CCR6−, Th0-polarized and CCR6+, Th17-polarized CD4 T cells from three donors was denatured and separated according to size by SDS-PAGE, transferred to a PVDF membrane, and then probed for the indicated RNase expression with anti-RNase antibodies. The loading control was glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (D) Th0- and Th17-polarized CD4 T cells were infected with HIVIIIB, washed, and then resuspended in medium containing the indicated RNases. Percent inhibition was calculated from the percentage of p24+ cells, as measured by flow cytometry.

Article Snippet: CD4 + T lymphocytes were isolated from human peripheral blood mononuclear cells by negative selection using the Easy Sep CD4 + human T cell enrichment kit, according to the manufacturer's protocol (Stem Cell Technologies Inc., Vancouver, BC, Canada).

Techniques: Comparison, Expressing, Inhibition, Flow Cytometry, Gene Expression, SDS Page, Membrane, Control, Infection